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Image Search Results
Journal: BMC Research Notes
Article Title: Expression and purification of hepatitis B surface antigen S from Escherichia coli ; a new simple method
doi: 10.1186/1756-0500-5-125
Figure Lengend Snippet: Cloning of the HBV S gene and 3'-UTR in the rapid cloning vector pCR2.1 . (A) An image of an agarose gel showing the MS-1-MS-2 PCR product. The * indicates the position of the PCR product. (B) An image of an agarose gel showing the supercoiled pCR2.1-HBsAg(S) and the * indicates the position of the plasmid. (C) An image of an agarose gel showing the KpnI/EcoRI digestion products of pCR2.1-HBsAg(S). The * indicates the position of the linearized plasmid and the ** indicates the position of the dropped insert. The left lane in each gel contains 1 kb DNA ladder (Promega, USA); the sizes of the respective bands is indicated on the left side of each panel.
Article Snippet: The insert cloned in pCR2.1-HBsAg(S) as described above was digested out using the restriction enzymes KpnI and
Techniques: Cloning, Plasmid Preparation, Agarose Gel Electrophoresis
Journal: BMC Research Notes
Article Title: Expression and purification of hepatitis B surface antigen S from Escherichia coli ; a new simple method
doi: 10.1186/1756-0500-5-125
Figure Lengend Snippet: Sub-cloning of the HBV S gene and 3'-UTR in the expression vector pRP265 . (A) An image of an agarose gel showing the supercoiled pRP265 and the * indicates the position of the plasmid. (B) An image of an agarose gel showing the KpnI/EcoRI digestion products of pRP265 and the * indicates the position of the linearized plasmid. (C) An image of an agarose gel showing the supercoiled pRP-HBsAg(S)-GST and the * indicates the position of the plasmid. (D) An image of an agarose gel showing the KpnI/EcoRI digestion products of pRP-HBsAg(S)-GST. The * indicates the position of the linearized plasmid and the ** indicates the position of the dropped insert. The left lane in each gel contains 1 kb DNA ladder (Promega, USA); the sizes of the respective bands is indicated on the left side of each panel.
Article Snippet: The insert cloned in pCR2.1-HBsAg(S) as described above was digested out using the restriction enzymes KpnI and
Techniques: Subcloning, Expressing, Plasmid Preparation, Agarose Gel Electrophoresis